Metode Analisis Pengendalian Mutu Stevia (Stevia rebaudiana Bertoni) : Systematic Literature Review Berdasarkan Parameter Kadar Rebaudioside A dan Stevioside Periode 2020-2025
DOI:
https://doi.org/10.51225/jps.v9i2.146Keywords:
kromatografi cair, pengendalian mutu, rebaudioside A, Stevia rebaudiana, UHPLC, liquid chromatography, quality controlAbstract
This study aims to systematically review analytical methods used for quality control of Stevia rebaudiana Bertoni during the 2020–2025 period and to identify the most reliable approaches for determining rebaudioside A and stevioside in dry extracts and commercial products. The review was conducted following the PRISMA 2020 guidelines, with literature searches performed in ScienceDirect, Springer Link, GARUDA, and Google Scholar using the keywords “Stevia rebaudiana,” “rebaudioside A,” “stevioside,” “quality control,” and “HPLC/UHPLC.” Selected studies were assessed for relevance, data completeness, and analytical validation parameters. The findings indicate that reverse phase high performance liquid chromatography (RP-HPLC) with UV detection remains the most widely applied method due to its reproducibility and suitability for routine quality control, while advanced techniques such as UHPLC combined with charged aerosol detection (CAD) or hyphenated with mass spectrometry provide higher sensitivity, selectivity, and shorter analysis time, particularly for complex matrices. These approaches ensure more reliable separation and quantification of rebaudioside A compared to non-chromatographic methods. Limitations include the small number of eligible studies and methodological heterogeneity, highlighting the need for harmonized analytical standardization and broader inter-laboratory validation in future research.
Author Biographies
Muhammad Raihan, Program Studi Farmasi, Fakultas Kedokteran, Universitas Lampung, Indonesia
Atri Sri Ulandari, Program Studi Farmasi, Fakultas Kedokteran, Universitas Lampung, Indonesia
Dosen Program Studi Farmasi, Fakultas Kedokteran, Universitas Lampung, Indonesia, yang berfokus pada bahan alam
Zulpakor Oktoba, Program Studi Farmasi, Fakultas Kedokteran, Universitas Lampung, Indonesia
Dosen di Program Studi Farmasi, Fakultas Kedokteran, Universitas Lampung, Indonesia, dengan fokus bahan alam
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Copyright (c) 2026 Muhammad Raihan, Atri Sri Ulandari, Zulpakor Oktoba
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