PENENTUAN KADAR FLAVONOID DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL 70% DAUN PUCUK MERAH (Syzygium myrtifolium Walp.) DENGAN METODE DPPH (2,2-diphenyl-1-picrylhydrazyl)

Dewi Nofita; Mia  Arifka; Miftahul  Khairi; Afi Salsabila  Putri

doi:10.51225/jps.v9i2.134

Authors

Dewi Nofita Sekolah Tinggi Sains Dwi Farma Bukittinggi
Mia Arifka Sekolah Tinggi Sains Dwi Farma Bukittinggi
Miftahul Khairi Sekolah Tinggi Sains Dwi Farma Bukittinggi
Afi Salsabila Putri Sekolah Tinggi Sains Dwi Farma Bukittinggi

DOI:

https://doi.org/10.51225/jps.v9i2.134

Keywords:

Antioksidan, DPPH, Flavonoid, IC₅₀, Pucuk merah, Syzygium myrtifolium

Abstract

Red tip (Syzygium myrtifolium Walp.) is an ornamental plant known to contain bioactive compounds, especially flavonoids, which have antioxidant potential. This study was conducted to determine the total flavonoid content and evaluate the antioxidant potential of 70% ethanol extract of pucuk merah leaves (Syzygium myrtifolium Walp.) using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Extraction was carried out by maceration using 70% ethanol with a yield of 32.02%. Flavonoid content was determined using the AlCl₃ colorimetric method based on a quercetin standard curve measured at a wavelength of 430 nm. The linear regression equation of the quercetin calibration curve was y = -0.0723 + 0.0090x with a coefficient of determination R² = 0.9076 and a correlation coefficient r = 0.9526. The total flavonoid content was 11.57 ± 0.0227 mg QE/g extract. The antioxidant activity assay using the DPPH method at 520 nm yielded an IC₅₀ value of 277.106 ppm, classified as weak antioxidant activity. This study demonstrates that 70% ethanol extract of red tip leaves contains flavonoid compounds and possesses antioxidant activity, albeit classified as weak.

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