PENGARUH LAMA PENYIMPANAN EKSTRAK DAUN JARAK PAGAR (Jatropha curcas L.) TERHADAP KADAR FLAVONOID TOTAL DAN AKTIVITAS ANTIOKSIDAN

Nina Nurhasanah Hasan; Sri Wahyuningsih

doi:10.51225/jps.v9i2.143

Authors

Nina Nurhasanah Hasan Universitas Megarezky
Sri Wahyuningsih Universitas Negeri Makassar https://orcid.org/0000-0002-7068-6912

DOI:

https://doi.org/10.51225/jps.v9i2.143

Keywords:

daun jarak pagar, soxhlet, flavonoid, antioksidan, lama penyimpanan ekstrak, antioxidant, jatropha curcas leaves, extract storage duration

Abstract

This laboratory experimental study with a quantitative approach aimed to evaluate the effect of storage duration on the total flavonoid content and antioxidant activity of ethanol extract of Jatropha curcas L. leaves. The extract was obtained using the Soxhlet extraction method with 96% ethanol as the solvent and stored at 4–8°C for 0, 3, 6, and 12 days. The total flavonoid content was analyzed using a UV-Vis spectrophotometer with quercetin as the standard, while antioxidant activity was assessed using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method by determining the IC₅₀ values. The results showed a decrease in total flavonoid content from 39,248 ± 0,091 mg QE/g on day 0 to 14,446 ± 0,060 mg QE/g on day 12. Antioxidant activity also decreased, as indicated by the increase in IC₅₀ value from 11,136 ± 0,013 ppm to 85,438 ± 2,005 ppm. Both flavonoid content and antioxidant activity declined progressively with longer storage duration. This study confirms that storage duration significantly affects the stability of flavonoid compounds and the antioxidant effectiveness of Jatropha curcas L. leaf extract.

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